Systemic exosomal siRNA delivery reduced alpha-synuclein aggregates in brains of transgenic mice
Identifieur interne : 000408 ( Main/Exploration ); précédent : 000407; suivant : 000409Systemic exosomal siRNA delivery reduced alpha-synuclein aggregates in brains of transgenic mice
Auteurs : J Mark Cooper [Royaume-Uni] ; Pb Oscar Wiklander [Suède] ; Joel Z. Nordin [Suède] ; Raya Al-Shawi [Royaume-Uni] ; Matthew J. Wood [Royaume-Uni] ; Mansi Vithlani [Royaume-Uni] ; Anthony H V. Schapira [Royaume-Uni] ; J Paul Simons [Royaume-Uni] ; Samir El-Andaloussi [Suède, Royaume-Uni] ; Lydia Alvarez-Erviti [Royaume-Uni]Source :
- Movement Disorders [ 0885-3185 ] ; 2014.
English descriptors
- KwdEn :
- Animals, Brain (metabolism), Cell Line, Tumor, Dendritic Cells (metabolism), Exosomes (physiology), Gene Expression Regulation (drug effects), Gene Expression Regulation (physiology), Genetic Vectors (genetics), Glycoproteins (administration & dosage), Glycoproteins (genetics), Humans, Mice, Mice, Inbred C57BL, Mice, Transgenic, Mutation (genetics), Neuroblastoma (pathology), Peptide Fragments (administration & dosage), Peptide Fragments (genetics), RNA, Messenger (metabolism), RNA, Small Interfering (administration & dosage), Time Factors, Transfection, Viral Proteins (administration & dosage), Viral Proteins (genetics), alpha-Synuclein (genetics), alpha-Synuclein (metabolism).
- MESH :
- chemical , administration & dosage : Glycoproteins, Peptide Fragments, RNA, Small Interfering, Viral Proteins.
- drug effects : Gene Expression Regulation.
- genetics : Genetic Vectors, Glycoproteins, Mutation, Peptide Fragments, Viral Proteins, alpha-Synuclein.
- metabolism : Brain, Dendritic Cells, RNA, Messenger, alpha-Synuclein.
- pathology : Neuroblastoma.
- physiology : Exosomes, Gene Expression Regulation.
- Animals, Cell Line, Tumor, Humans, Mice, Mice, Inbred C57BL, Mice, Transgenic, Time Factors, Transfection.
Abstract
Alpha-synuclein (α-Syn) aggregates are the main component of Lewy bodies, which are the characteristic pathological feature in Parkinson's disease (PD) brain. Evidence that α-Syn aggregation can be propagated between neurones has led to the suggestion that this mechanism is responsible for the stepwise progression of PD pathology. Decreasing α-Syn expression is predicted to attenuate this process and is thus an attractive approach to delay or halt PD progression. We have used α-Syn small interfering RNA (siRNA) to reduce total and aggregated α-Syn levels in mouse brains. To achieve widespread delivery of siRNAs to the brain we have peripherally injected modified exosomes expressing Ravies virus glycoprotein loaded with siRNA. Normal mice were analyzed 3 or 7 days after injection. To evaluate whether this approach can decrease α-Syn aggregates, we repeated the treatment using transgenic mice expressing the human phosphorylation-mimic S129D α-Syn, which exhibits aggregation. In normal mice we detected significantly reduced α-Syn messenger RNA (mRNA) and protein levels throughout the brain 3 and 7 days after treatment with RVG-exosomes loaded with siRNA to α-Syn. In S129D α-Syn transgenic mice we found a decreased α-Syn mRNA and protein levels throughout the brain 7 days after injection. This resulted in significant reductions in intraneuronal protein aggregates, including in dopaminergic neurones of the substantia nigra. This study highlights the therapeutic potential of RVG-exosome delivery of siRNA to delay and reverse brain α-Syn pathological conditions. © 2014 The Authors.
Url:
DOI: 10.1002/mds.25978
PubMed: 25112864
PubMed Central: 4204174
Affiliations:
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Nordin</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Department of Laboratory Medicine, Karolinska Institutet</institution><addr-line>Huddinge, Sweden</addr-line></nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>Huddinge</wicri:regionArea></affiliation></author><author><name sortKey="Al Shawi, Raya" sort="Al Shawi, Raya" uniqKey="Al Shawi R" first="Raya" last="Al-Shawi">Raya Al-Shawi</name><affiliation wicri:level="1"><nlm:aff id="au3"><institution>Centre for Biomedical Science, Division of Medicine, UCL</institution><addr-line>London, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>London</wicri:regionArea></affiliation></author><author><name sortKey="Wood, Matthew J" sort="Wood, Matthew J" uniqKey="Wood M" first="Matthew J" last="Wood">Matthew J. Wood</name><affiliation wicri:level="1"><nlm:aff id="au4"><institution>Department of Physiology, Anatomy and Genetics, University of Oxford</institution><addr-line>Oxford, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Oxford</wicri:regionArea></affiliation></author><author><name sortKey="Vithlani, Mansi" sort="Vithlani, Mansi" uniqKey="Vithlani M" first="Mansi" last="Vithlani">Mansi Vithlani</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Department of Clinical Neuroscience, Institute of Neurology, University College London</institution><addr-line>London, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>London</wicri:regionArea></affiliation></author><author><name sortKey="Schapira, Anthony H V" sort="Schapira, Anthony H V" uniqKey="Schapira A" first="Anthony H V" last="Schapira">Anthony H V. Schapira</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Department of Clinical Neuroscience, Institute of Neurology, University College London</institution><addr-line>London, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>London</wicri:regionArea></affiliation></author><author><name sortKey="Simons, J Paul" sort="Simons, J Paul" uniqKey="Simons J" first="J Paul" last="Simons">J Paul Simons</name><affiliation wicri:level="1"><nlm:aff id="au3"><institution>Centre for Biomedical Science, Division of Medicine, UCL</institution><addr-line>London, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>London</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="au5"><institution>Wolfson Drug Discovery Unit, Centre for Amyloidosis and Acute Phase Proteins, UCL</institution><addr-line>London, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>London</wicri:regionArea></affiliation></author><author><name sortKey="El Andaloussi, Samir" sort="El Andaloussi, Samir" uniqKey="El Andaloussi S" first="Samir" last="El-Andaloussi">Samir El-Andaloussi</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Department of Laboratory Medicine, Karolinska Institutet</institution><addr-line>Huddinge, Sweden</addr-line></nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>Huddinge</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="au4"><institution>Department of Physiology, Anatomy and Genetics, University of Oxford</institution><addr-line>Oxford, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Oxford</wicri:regionArea></affiliation></author><author><name sortKey="Alvarez Erviti, Lydia" sort="Alvarez Erviti, Lydia" uniqKey="Alvarez Erviti L" first="Lydia" last="Alvarez-Erviti">Lydia Alvarez-Erviti</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Department of Clinical Neuroscience, Institute of Neurology, University College London</institution><addr-line>London, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>London</wicri:regionArea></affiliation></author></analytic><series><title level="j">Movement Disorders</title><idno type="ISSN">0885-3185</idno><idno type="eISSN">1531-8257</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Brain (metabolism)</term><term>Cell Line, Tumor</term><term>Dendritic Cells (metabolism)</term><term>Exosomes (physiology)</term><term>Gene Expression Regulation (drug effects)</term><term>Gene Expression Regulation (physiology)</term><term>Genetic Vectors (genetics)</term><term>Glycoproteins (administration & dosage)</term><term>Glycoproteins (genetics)</term><term>Humans</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Mice, Transgenic</term><term>Mutation (genetics)</term><term>Neuroblastoma (pathology)</term><term>Peptide Fragments (administration & dosage)</term><term>Peptide Fragments (genetics)</term><term>RNA, Messenger (metabolism)</term><term>RNA, Small Interfering (administration & dosage)</term><term>Time Factors</term><term>Transfection</term><term>Viral Proteins (administration & dosage)</term><term>Viral Proteins (genetics)</term><term>alpha-Synuclein (genetics)</term><term>alpha-Synuclein (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Glycoproteins</term><term>Peptide Fragments</term><term>RNA, Small Interfering</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gene Expression Regulation</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genetic Vectors</term><term>Glycoproteins</term><term>Mutation</term><term>Peptide Fragments</term><term>Viral Proteins</term><term>alpha-Synuclein</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Brain</term><term>Dendritic Cells</term><term>RNA, Messenger</term><term>alpha-Synuclein</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Neuroblastoma</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Exosomes</term><term>Gene Expression Regulation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line, Tumor</term><term>Humans</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Mice, Transgenic</term><term>Time Factors</term><term>Transfection</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Alpha-synuclein (α-Syn) aggregates are the main component of Lewy bodies, which are the characteristic pathological feature in Parkinson's disease (PD) brain. Evidence that α-Syn aggregation can be propagated between neurones has led to the suggestion that this mechanism is responsible for the stepwise progression of PD pathology. Decreasing α-Syn expression is predicted to attenuate this process and is thus an attractive approach to delay or halt PD progression. We have used α-Syn small interfering RNA (siRNA) to reduce total and aggregated α-Syn levels in mouse brains. To achieve widespread delivery of siRNAs to the brain we have peripherally injected modified exosomes expressing Ravies virus glycoprotein loaded with siRNA. Normal mice were analyzed 3 or 7 days after injection. To evaluate whether this approach can decrease α-Syn aggregates, we repeated the treatment using transgenic mice expressing the human phosphorylation-mimic S129D α-Syn, which exhibits aggregation. In normal mice we detected significantly reduced α-Syn messenger RNA (mRNA) and protein levels throughout the brain 3 and 7 days after treatment with RVG-exosomes loaded with siRNA to α-Syn. In S129D α-Syn transgenic mice we found a decreased α-Syn mRNA and protein levels throughout the brain 7 days after injection. This resulted in significant reductions in intraneuronal protein aggregates, including in dopaminergic neurones of the substantia nigra. This study highlights the therapeutic potential of RVG-exosome delivery of siRNA to delay and reverse brain α-Syn pathological conditions. © 2014 The Authors. <italic>Movement</italic> Disorders published by Wiley Periodicals, Inc. on behalf of International Parkinson and Movement Disorder Society.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Lee, Vm" uniqKey="Lee V">VM Lee</name></author><author><name sortKey="Trojanowski, Jq" uniqKey="Trojanowski J">JQ Trojanowski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Braak, H" uniqKey="Braak H">H Braak</name></author><author><name sortKey="Del Tredici, K" uniqKey="Del Tredici K">K Del Tredici</name></author><author><name sortKey="Rub, U" uniqKey="Rub U">U Rüb</name></author><author><name sortKey="De Vos, Ra" uniqKey="De Vos R">RA de Vos</name></author><author><name sortKey="Jansen Steur, En" uniqKey="Jansen Steur E">EN Jansen Steur</name></author><author><name sortKey="Braak, E" uniqKey="Braak E">E Braak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jellinger, K" uniqKey="Jellinger K">K Jellinger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lewis, J" uniqKey="Lewis J">J Lewis</name></author><author><name sortKey="Melrose, H" uniqKey="Melrose H">H Melrose</name></author><author><name sortKey="Bumcrot, D" uniqKey="Bumcrot D">D Bumcrot</name></author><author><name sortKey="Hope, A" uniqKey="Hope A">A Hope</name></author><author><name sortKey="Zehr, C" uniqKey="Zehr C">C Zehr</name></author><author><name sortKey="Lincoln, S" uniqKey="Lincoln S">S Lincoln</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maysinger, D" uniqKey="Maysinger D">D Maysinger</name></author><author><name sortKey="Morinville, A" uniqKey="Morinville A">A Morinville</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Coune, Pg" uniqKey="Coune P">PG Coune</name></author><author><name sortKey="Schneider, Bl" uniqKey="Schneider B">BL Schneider</name></author><author><name sortKey="Aebischer, P" uniqKey="Aebischer P">P Aebischer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name></author><author><name sortKey="Pardridge, Wm" uniqKey="Pardridge W">WM Pardridge</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alvarez Erviti, L" uniqKey="Alvarez Erviti L">L Alvarez-Erviti</name></author><author><name sortKey="Seow, Y" uniqKey="Seow Y">Y Seow</name></author><author><name sortKey="Yin, H" uniqKey="Yin H">H Yin</name></author><author><name sortKey="Betts, C" uniqKey="Betts C">C Betts</name></author><author><name sortKey="Lakhal, S" uniqKey="Lakhal S">S Lakhal</name></author><author><name sortKey="Wood, Mj" uniqKey="Wood M">MJ Wood</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Valadi, H" uniqKey="Valadi H">H Valadi</name></author><author><name sortKey="Ekstrom, K" uniqKey="Ekstrom K">K Ekström</name></author><author><name sortKey="Bossios, A" uniqKey="Bossios A">A Bossios</name></author><author><name sortKey="Sjostrand, M" uniqKey="Sjostrand M">M Sjöstrand</name></author><author><name sortKey="Lee, Jj" uniqKey="Lee J">JJ Lee</name></author><author><name sortKey="Lotvall, Jo" uniqKey="Lotvall J">JO Lötvall</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alvarez Erviti, L" uniqKey="Alvarez Erviti L">L Alvarez-Erviti</name></author><author><name sortKey="Seow, Y" uniqKey="Seow Y">Y Seow</name></author><author><name sortKey="Schapira, Ah" uniqKey="Schapira A">AH Schapira</name></author><author><name sortKey="Rodriguez Oroz, Mc" uniqKey="Rodriguez Oroz M">MC Rodriguez-Oroz</name></author><author><name sortKey="Obeso, Ja" uniqKey="Obeso J">JA Obeso</name></author><author><name sortKey="Cooper, Jm" uniqKey="Cooper J">JM Cooper</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Azeredo Da Silveira, S" uniqKey="Azeredo Da Silveira S">S Azeredo da Silveira</name></author><author><name sortKey="Schneider, Bl" uniqKey="Schneider B">BL Schneider</name></author><author><name sortKey="Cifuentes Diaz, C" uniqKey="Cifuentes Diaz C">C Cifuentes-Diaz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Anderson, Jp" uniqKey="Anderson J">JP Anderson</name></author><author><name sortKey="Walker, De" uniqKey="Walker D">DE Walker</name></author><author><name sortKey="Goldstein, Jm" uniqKey="Goldstein J">JM Goldstein</name></author><author><name sortKey="De Laat, R" uniqKey="De Laat R">R de Laat</name></author><author><name sortKey="Banducci, K" uniqKey="Banducci K">K Banducci</name></author><author><name sortKey="Caccavello, Rj" uniqKey="Caccavello R">RJ Caccavello</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fleming, Sm" uniqKey="Fleming S">SM Fleming</name></author><author><name sortKey="Salcedo, J" uniqKey="Salcedo J">J Salcedo</name></author><author><name sortKey="Fernagut, Po" uniqKey="Fernagut P">PO Fernagut</name></author><author><name sortKey="Rockenstein, E" uniqKey="Rockenstein E">E Rockenstein</name></author><author><name sortKey="Masliah, E" uniqKey="Masliah E">E Masliah</name></author><author><name sortKey="Levine, Ms" uniqKey="Levine M">MS Levine</name></author><author><name sortKey="Chesselet, Mf" uniqKey="Chesselet M">MF Chesselet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Singleton, Ab" uniqKey="Singleton A">AB Singleton</name></author><author><name sortKey="Farrer, M" uniqKey="Farrer M">M Farrer</name></author><author><name sortKey="Johnson, J" uniqKey="Johnson J">J Johnson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chartier Harlin, Mc" uniqKey="Chartier Harlin M">MC Chartier-Harlin</name></author><author><name sortKey="Kachergus, J" uniqKey="Kachergus J">J Kachergus</name></author><author><name sortKey="Roumier, C" uniqKey="Roumier C">C Roumier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Danzer, Km" uniqKey="Danzer K">KM Danzer</name></author><author><name sortKey="Haasen, D" uniqKey="Haasen D">D Haasen</name></author><author><name sortKey="Karow, Ar" uniqKey="Karow A">AR Karow</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Abeliovich, A" uniqKey="Abeliovich A">A Abeliovich</name></author><author><name sortKey="Schmitz, Y" uniqKey="Schmitz Y">Y Schmitz</name></author><author><name sortKey="Fari As, I" uniqKey="Fari As I">I Fariñas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Luk, Kc" uniqKey="Luk K">KC Luk</name></author><author><name sortKey="Kehm, V" uniqKey="Kehm V">V Kehm</name></author><author><name sortKey="Carroll, J" uniqKey="Carroll J">J Carroll</name></author><author><name sortKey="Zhang, B" uniqKey="Zhang B">B Zhang</name></author><author><name sortKey="O Brien, P" uniqKey="O Brien P">P O'Brien</name></author><author><name sortKey="Trojanowski, Jq" uniqKey="Trojanowski J">JQ Trojanowski</name></author><author><name sortKey="Lee, Vm" uniqKey="Lee V">VM Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Recasens, A" uniqKey="Recasens A">A Recasens</name></author><author><name sortKey="Dehay, B" uniqKey="Dehay B">B Dehay</name></author><author><name sortKey="Bove, J" uniqKey="Bove J">J Bové</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dawson, Tm" uniqKey="Dawson T">TM Dawson</name></author><author><name sortKey="Ko, Hs" uniqKey="Ko H">HS Ko</name></author><author><name sortKey="Dawson, Vl" uniqKey="Dawson V">VL Dawson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fujiwara, H" uniqKey="Fujiwara H">H Fujiwara</name></author><author><name sortKey="Hasegawa, M" uniqKey="Hasegawa M">M Hasegawa</name></author><author><name sortKey="Dohmae, N" uniqKey="Dohmae N">N Dohmae</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fujiwara, H" uniqKey="Fujiwara H">H Fujiwara</name></author><author><name sortKey="Hasegawa, M" uniqKey="Hasegawa M">M Hasegawa</name></author><author><name sortKey="Dohmae, N" uniqKey="Dohmae N">N Dohmae</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Febbraro, F" uniqKey="Febbraro F">F Febbraro</name></author><author><name sortKey="Sahin, G" uniqKey="Sahin G">G Sahin</name></author><author><name sortKey="Farran, A" uniqKey="Farran A">A Farran</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yamamoto, A" uniqKey="Yamamoto A">A Yamamoto</name></author><author><name sortKey="Lucas, Jj" uniqKey="Lucas J">JJ Lucas</name></author><author><name sortKey="Hen, R" uniqKey="Hen R">R Hen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Difiglia, M" uniqKey="Difiglia M">M DiFiglia</name></author><author><name sortKey="Sena Esteves, M" uniqKey="Sena Esteves M">M Sena-Esteves</name></author><author><name sortKey="Chase, K" uniqKey="Chase K">K Chase</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Royaume-Uni</li><li>Suède</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Cooper, J Mark" sort="Cooper, J Mark" uniqKey="Cooper J" first="J Mark" last="Cooper">J Mark Cooper</name></noRegion><name sortKey="Al Shawi, Raya" sort="Al Shawi, Raya" uniqKey="Al Shawi R" first="Raya" last="Al-Shawi">Raya Al-Shawi</name><name sortKey="Alvarez Erviti, Lydia" sort="Alvarez Erviti, Lydia" uniqKey="Alvarez Erviti L" first="Lydia" last="Alvarez-Erviti">Lydia Alvarez-Erviti</name><name sortKey="El Andaloussi, Samir" sort="El Andaloussi, Samir" uniqKey="El Andaloussi S" first="Samir" last="El-Andaloussi">Samir El-Andaloussi</name><name sortKey="Schapira, Anthony H V" sort="Schapira, Anthony H V" uniqKey="Schapira A" first="Anthony H V" last="Schapira">Anthony H V. Schapira</name><name sortKey="Simons, J Paul" sort="Simons, J Paul" uniqKey="Simons J" first="J Paul" last="Simons">J Paul Simons</name><name sortKey="Simons, J Paul" sort="Simons, J Paul" uniqKey="Simons J" first="J Paul" last="Simons">J Paul Simons</name><name sortKey="Vithlani, Mansi" sort="Vithlani, Mansi" uniqKey="Vithlani M" first="Mansi" last="Vithlani">Mansi Vithlani</name><name sortKey="Wood, Matthew J" sort="Wood, Matthew J" uniqKey="Wood M" first="Matthew J" last="Wood">Matthew J. Wood</name></country><country name="Suède"><noRegion><name sortKey="Wiklander, Pb Oscar" sort="Wiklander, Pb Oscar" uniqKey="Wiklander P" first="Pb Oscar" last="Wiklander">Pb Oscar Wiklander</name></noRegion><name sortKey="El Andaloussi, Samir" sort="El Andaloussi, Samir" uniqKey="El Andaloussi S" first="Samir" last="El-Andaloussi">Samir El-Andaloussi</name><name sortKey="Nordin, Joel Z" sort="Nordin, Joel Z" uniqKey="Nordin J" first="Joel Z" last="Nordin">Joel Z. 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